A Great Read and Reference, "The Oregon Bigfoot Highway"

The Oregon Bigfoot Highway
Copyright © 2015 by Joe Beelart and Cliff Olson
ISBN 979-0-69238-081-9
Willamette City Press, LLC
331 numbered pages, 15 additional pages
Available from Amazon.com  $19.99
See also Oregonbigfoothighway.com for more information, including an interactive map.

I met Joe Beelart at the Beachfoot 2017 Conference in July, and was warmly treated by him and his wife, Sharon.  Later he sent me his latest book, The Oregon Bigfoot Highway, which I devoured in short order.  I have never read a more detailed and precisely written book on how to, what to, where to, and why to find bigfoot.  The authors have graciously shared experiences so that others may have some too, in refreshing contrast to many bigfoot researchers who conceal their details in hopes of being "the first." 

The book covers a 70 mile stretch of the Oregon Scenic Byway No. 5, from Estacada to Detroit (OR), plus numerous intersecting side roads and forest roads where the action takes place.  The journey follows OR 224 (The Clackamas Rd.) along the Clackamas River out of Estacada, and then finishes on NFD 46 (Breitenbush Rd.) along the Breitenbush River to Detroit.   Organized into six areas, the journey describes 31 sightings going back to 1924, 43 quality track finds going back to 1911, and 69 probable bigfoot-related incidents (such as calls, rock throwing, and shelters), all individually numbered, cross referenced, and indexed.   You will not find a comparably complete and well documented account of real experiences, including many of the authors’ own, plus well researched media reports and personal interviews.

The book is well illustrated with 118 pictures (most by Joe and Sharon) and 13 custom maps, the latter thanks to Sharon Beelart.  GPS coordinates are also given for points of interest.  You are easily led to the “hot spots.”

Interspersed throughout the book are safety warnings, conservation ethics, fishing hole descriptions, useful tips (e.g. what to bring, where to park safely), and other, often humorous, little tidbits.  The Clackamas is a National Scenic River, a steelhead fishery, and a cherished place to visit, without or without bigfoot.   I plan to do so at the first opportunity, clutching this great book in my hands.
  

Die Tiefe: A Great Blog for Bigfoot Issues

Christoph Kummer is a journalist who writes for various newspapers in Switzerland.  His blog, "Die Tiefe: Der Wahrheit auf der Spur..." (The Deep:  Truth on Track) is an online newspaper that deals with mysteries and controversies of all kinds ( http://www.dietiefe.com/ ).  So far, the main themes have been 9/11 and bigfoot.  Articles are in English and German.  He has covered the bigfoot phenomenon historically and has interviewed a number of involved researchers, including me.  So, of course, I encourage you to check out my recent interview with Chris on "Die Tiefe" to get an overview of what went wrong with the Ketchum bigfoot DNA study.  A number of readers have said that it was understandable to the layman.  Chris' poignant and to-the-point questions have made this a very clear expose.   

Unusual Primate Mutations in Some Ketchum mtDNA Samples

“Shouldn’t we not throw the baby out with the bathwater?…Is there anything we can salvage from the Ketchum study?”     A Facebook Post

In my past work I have tried to treat each Ketchum et al. DNA sequence independently, and not to generalize without evidence. Sometimes a tentative overall conclusion is suggested. Here we look at some mtDNA sequences with common rare (human) mutations which are much more prevalent in other primates. From everything I have learned, these several samples hold the most promise for actually originating from an unknown human-like primate. This is a preliminary report, mostly just observations and suggestions for further study. Your comments are especially welcome here.

   

Comparisons between mtDNA extra mutations (i.e. those not found in the nearest haplogroup) revealed some unlikely coincidences. Table 1 shows these. Sample 26 has been shown to be a black bear [2a,b] from its nuclear DNA sequence taken from the original Ketchum paper [1], but the mtDNA results were much closer to human, though outside the normal range of number of extra mutations. [3] Although the Ketchum et al. conclusion was that sasquatch is a hybrid of an unknown primate male and a modern human female, human mtDNA in this sample has been widely attributed to contamination. [4, 7] ES-2 was discovered unlabeled below and to the right of other entries in the Ketchum Supplementary Data 2 and is not listed in the sample Table 1 there [1]. Sample 24 failed to produce human Amel X and Y STRs and had a very low 63% of 2.5 M human SNPs. Samples 29 and 138 are not included in any nDNA analyses in the Ketchum paper. Sample 28 appears in Table 5 of ref. [1], where it shows human STRs in all but one locus (D3S1358) of sixteen total microsatellite loci. It also matched nine of ten human SNP sites, with a heteroplasmic mutation at the tenth (478RHC) on the MC1R gene (Table 6, ref. [1]). However, this sample failed to sequence at Amel X and at AmelY exons 1, 3, and 8, while showing human sequences at exons 2 and 4/5 (Table 4, ref. [1]). Overall Sample 28 is the most human-like of those samples which were put through all of these nDNA tests.

Referring to Table 1, samples S1 and ES-2 have identical mtDNA sequences and the same two extra mutations. C152T is common in humans, but G7332c is not found among the nearly 20,000 human mtDNA sequences in the Nucleotide database. Further, it was not found in any other primates, though G7332A had a few examples. Since we know nothing of the provenance of ES-2 and because it is identical to S1, it would be of great interest if Ketchum et al. would identify this sample. However, the most significant similarity among the samples in Table 1 is the occurrence of 7852A, 9083C, and 13209T in samples S24, S26 (less 7852A), S28, S29, and S138. These mutations are rare in both the NCBI Nucleotide database and the Phylotree of mtDNA mutations [6]. In fact, none of the nearly 20,000 complete human mtDNA genomes in the database have more than one of these mutations, i.e. none have two or three. These five samples were collected by different teams in three widely separated locations, CA, NM, and BC. Ketchum, et al.[1] maintain that all sample collectors and laboratory personnel were excluded from possible contamination based on their individual mtDNA profiles.

Fig. 1 shows percentages of each primate group possessing each of these rare human mutations. Percentages are based on all database entries (number in parentheses) for each group in GenBank(R) - Nucleotide database, without regard for species duplicates. The groups collectively include all living primates: chimpanzees (two species), gorilla (several subspecies), orangutan (two species),
gibbons, tarsiers, Old World (OW) monkeys, New World (NW) monkeys, lemurs, Lorisiformes (loris’s and galagos), and Chiromyiformes (aye-ayes). None of these extra mutations occurred in any Neanderthal (9 database examples), Heidelberg (1), or Denisovan (2) sequences, and are therefore not plotted in Fig. 1. However, these small numbers of database examples may not be representative of these populations. Full taxonomy of these families is found in ref. [5].that these three mutations, plus C9195T, are much more common in nonhuman primates, increasing generally with increasing taxonomic/genetic distance from human [5]. C6571T was only found in Old World monkeys. C3626T was not found in other primates.

Nothing definite can be concluded from these limited data, especially when considering the relatively low number of database entries for some of the primate families in Fig. 1. Some intriguing questions might be explored further by collection of more DNA samples from the same geographical areas of CA, NM, and BC, along with photographic or video documentation:

1.  Could S26 (the black bear) be contaminated with sasquatch mtDNA? In which case, it might be the result of a fight between bears over a sasquatch carcass. [7]

2. Why does S26 have so many (16) extra mutations? (so many that a haplogroup cannot be uniquely determined). [3] Phylotrees produced by two different methods did not agree. [4]

3. Could S24, S28, S29, and S138 actually be sasquatch samples with a vestige of nonhuman primate mutations, either through parallel or reverse evolution? The random occurrence of three rare mutations (7852A, 9083C, and 13209T) in all of these samples is a statistically improbable coincidence. [See NOTE below] They must be related somehow. Importantly, all four samples differ by only a few, mostly heteroplasmic, mutations. A common human contamination is unlikely, given the rarity of these mutations in the human genome (Table 1, Fig. 1), especially in combination. Even two human contaminants with these combined mutations seem unlikely (no GenBank(R) sequences had even two of these mutations). Even more remarkable are the identical sequences of S29 and S138, which have in addition to these mutations three additional rare heteroplasmic mutations (C3626Y, C6571Y, and C9195Y) in common. Sequencing errors would not likely be at the same three positions in two independent samples. Both of these samples are from British Columbia and were collected by the same people (ref. 1,Table 1), whose mtDNA was found not to be in these samples [1]. These two samples may be from a single sasquatch or closely related individuals.

I openly encourage more field work and laboratory analysis of samples from these regions of OK, CA, NM, and BC.

       
NOTE

The occurrences of 7852A, 9083C, and 13209T are 27, 20, and 7 respectively in 19988 human database entries. The probability of all three occurring in the same individual is:


(27/19988)*(20/19988)*(7/19988) = 4.73 x 10 exp -10.

The probability of these three mutations occurring in any four randomly selected individuals is:

(4.73 x 10 exp -10) exp 4 = 5.02 x 10 exp -38.

REFERENCES

[1] Ketchum, M. S. et al., Novel North American Hominins: Next Generation Sequencing of
Three Whole Genomes and Associated Studies. DeNovo, 2013, 1:1, Online only: http://sasquatchgenomeproject.org/sasquatch_genome_project_002.htm

[2a] Hart, H. V., Methodology and New Metrics for Distinguishing Related Species from
Incomplete nuDNA., Paper 1 at right (and related blogs)http://www.bigfootclaims.blogspot.com

[2b] Hart, H. V., Not Finding Bigfoot in DNA. Journal of Cryptozoology 4: 39-51.

[3] Hart, H. V., “But the mtDNA Sequences are all Human…” Really?, Paper 2 at right: http://www.bigfootclaims.blogspot.com

[4] Hart, H. V., More Inconsistencies and Evidence for Contamination in Ketchum et al.
Supplementary Figures, June 17, 2016, this blogsite.

[5] National Center for Biotechnology, http://www.ncbi.nlm.nih.gov/guide/taxonomy/

[6] van Oven, M., Revision of the mtDNA Tree and Corresponding Haplogroup Nomenclature. Proc. Natl. Acad. Sci. USA, 2010, 107(11), E38-E39. http://dx.doi.org/10.1073/pnas.0915120107

[7] Hart, H. V., Ketchum Sample 26, The Smeja Kill: Independent Lab Reports, November 26, 2014, this blogsite. http://www.bigfootclaims.blogspot.com/2014/11/sample-26-smeja-kill-independent-lab.html

FIG. 1






Numbers in parentheses for each group are the number of database entries in GenBank(R), which include duplicate entries for some species.



Two New Peer-reviewed Papers are at Odds with Ketchum et al. Conclusions

“The above commonly reported traits, as well as other scientific evidence lending credence to the existence of Sasquatch, have been thoroughly researched and documented in both books and in peer reviewed manuscripts. refs.4-13”  (Ketchum et al., 2013)

Peer review is the process by which journal articles are reviewed by experts prior to publication.  Reviewers are selected by the editor of the journal for their proven expertise in the relevant area, and their identity is kept anonymous. Their criticisms and suggestions are forwarded to the author for consideration and possible revision of the manuscript.  It’s not a perfect system, but it greatly reduces the number of scurrilous publications, honest errors, and unclear narratives.  Recall that Ketchum et al. (2013) failed peer review in two journals before being self-published.

Recently I had published two, peer-reviewed papers (Hart, 2016a, 2016b).  “Not Finding Bigfoot in DNA” made it to Volume 4 (pp. 39-51) of The Journal of Cryptozoology, a publication of the Center for Fortean Zoology, edited by Dr. Karl Shuker.  Zoologist Shuker is well known for his many books and blog articles on cryptozoology – the study of animals not (yet) proven to exist by science.  My paper addresses the three nuclear DNA sequences published by Ketchum et al. (2013), said by them to be “a novel mosaic pattern of nuclear DNA comprising novel sequences that are related to primates interspersed with sequences that are closely homologous to humans.“  As proven in my previous blogs, this claim is false:  the sequences are from a bear (S26), a human (S31), and a dog (S140), with no significant traces of other primates.  I review the five different approaches to producing realistic phylotrees* of S26 and S140 that clearly show the samples are related to bears and dogs, respectively.   In contrast, Ketchum phylotrees for S31 (their Supp. Fig. 5) and S140 (their Supp. Fig. 6) showed homology to mice, chicken, and fish, not at all in support of their conclusion above.   Volume 4 of the Journal of Crypozoology is available from Amazon.com.
 
My second paper, “DNA as Evidence for the Existence of Relict Hominoids,” is a review of known publications related to DNA of purported cryptid hominoids, and can be downloaded free from:  https://www.isu.edu/media/libraries/rhi/research-papers/HART-DNA-Evidence.pdf, the Relict Hominoid Inquiry website of Prof. Jeff Meldrum of Idaho State.  Meldrum’s specialty is anatomy, especially primate bipedal motion, and he has studied hundreds of purported sasquatch footprint casts in great detail, the great majority of which he sees as evidence for the existence of a large North American primate.

Finally, one of the criteria for publication in peer-reviewed journals is that the references are authentic and support the statements in the text to which they are appended.  Some journals require that the author certify this.  Padding of references to give the appearance of command of the literature is explicitly discouraged.  References 5 (Malinkovitch et al., 2004) and 6 (Coltman and Davis, 2006) of Ketchum et al. (2013), both with titles in apparent support of the Ketchum et al. (2013) claim quoted at the beginning of this blog, are anything but supportive, if one bothers to read them.  Both references are reviewed in my second paper.

The Milinkovitch et al. (2004) paper has an admitted April Fool’s joke title.  The Himalayan hair sample was found to be from a horse, nothing close to a primate.  Their phylotree shows this clearly.

Coltman and Davis (2006) matched the DNA of a Yukon hair to the American bison exactly, in spite of their tongue-in-cheek title.   Their phylotree of related ungulates supports their conclusion.

Both of these papers are good examples of how an unknown DNA sample should be analyzed without bias.  Read your references, Melba.  You might learn something. 

 *  A phylotree is a DNA-based evolutionary tree of life with a topology determined by degree of match (distance – or % of matching base pairs in homologous DNA sequences) between the various species in the branches.  A phylotree is produced from the results of a DNA search, for example using BLAST® as both Ketchum et al. and I did.

REFERENCES  (Unpadded)

Coltman, D. and Davis, C. (2006) “Molecular cryptozoology meets the Sasquatch.“ TRENDS in Ecology and Evolution 21(2): 60–61.

Hart, H. V. (2016a)  “Not Finding Bigfoot in DNA.”  Journal of Cryptozoology 4: 39-51.

Hart, H. V. (2016b)  “DNA as Evidence for the Existence of Relict Hominoids” Relict Hominoid Inquiry 5: 8-31.  
https://www.isu.edu/media/libraries/rhi/research-papers/HART-DNA-Evidence.pdf

Ketchum, M. S. et al. (2013). “Novel North American Hominins: Next Generation Sequencing of Three Whole Genomes and Associated Studies.“ DeNovo,  1 (1):  Online only:  http://sasquatchgenomeproject.org/sasquatch_genome_project_002.htm


Milinkovitch, M. C. et al. (2004) “Molecular phylogenetic analyses indicate extensive morphological convergence between the ‘yeti’  and primates.” Molecular Phylogenetics and Evolution 31: 1–3.